Coding

Part:BBa_K4853001:Design

Designed by: Bhavya Guduru   Group: iGEM23_Virginia   (2023-10-12)


nisin dehydratase (1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2990
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 107


Design Notes

Sequence was codon optimized for E. coli BL21(DE3) using IDT Codon Optimization Tool.

Source

This gene is originally found in Lactococcus lactis. The sequence was found through the NCBI nucleotide database1.


1 Lactococcus lactis strain N8 chromosome, complete genome - nucleotide - NCBI. National Center for Biotechnology Information. U.S. National Library of Medicine.

References